Unsexed pupae was kept for the a cage so that grownups so you’re able to arise and you may mate

So far, little is well known from the potato odour characteristics to have https://datingmentor.org/local-hookup/canberra/ T. solanivora location regarding lover and you can/or oviposition web site. Interestingly, Bosa et al. (2011) stated that virgin ladies was much more keen on rose odour than to odours of entire bush or even tubers, within the a keen olfactometer decide to try. Because the appeal in order to herbs certainly virgin people moths can be associated with venue off feeding internet sites and never from oviposition websites (Cunningham ainsi que al. 2006; Saveer et al. 2012), the fresh new part away from potato flower odour throughout the servers locating means out-of T. solanivora must be searched.

Potato gives off unpredictable ingredients which might be species certain and others one to suggest the quality as well as the phenological phase of your own flowers (Bolter et al. 1997; Agelopoulos et al. 2000; Karlsson ainsi que al. 2009, 2013). All of the potato plant structures, potato foliage, tubers, and you can vegetation create compounds, many of which is antennal energetic so you’re able to T. solanivora (Karlsson et al. 2009). Semi-job trapping assays showed that the fresh material methyl phenylacetate (MPA) released regarding potato plants for the highest number and you may potato tubers for the small quantity attracts T. solanivora. However, MPA in combination with a premier concentration of six-methyl-5-hepten-2-one to (sulcatone), a substance used in tubers, decreases the destination (Bosa et al. 2011).

The goal of today’s studies were to characterize this new cues used by T. solanivora to get its host. I hypothesize one to, because tuber creation and you may flowering peak occur on top of that during the potato plant life, potato rose odour you certainly will guide female to find an enthusiastic oviposition web site. To evaluate that it theory, actions assays have been presented to observe impulse from T. solanivora so you can artificial blends, identified out-of and you can representing additional potato bush formations. The new mating standing dictate on behaviour is actually tested also because the significance of private substances regarding most attractive blend.

Experimental pests

A laboratory colony of your own Guatemalan potato moth T. solanivora, originating from the Colombian Enterprise to possess Farming Lookup (Corpoica), was built. The latest colony try interbred frequently having crazy Colombian moths and you may try reared to your potato tubers in containment business on SLU, Sweden. Bugs had been was able inside 60 ± 10% RH and you may 18 ± 2 °C around a photoperiod out-of L16:8D. People getting electrophysical try out have been kept in Plexiglas cages (33 ? 33 ? 33 cm 3 ) and you may given which have ten% honey provider, while grownups towards olfactometer assays was fed just with drinking water twenty four h ahead of studies.


Man-made ingredients (Table 1 ) have been checked-out during the an amount–reaction sort out electroantennographic recording technique (EAG) more four requests away from magnitude: 0.01, 0.step one, 1 and you may ten ?g ?l ?1 . The newest substances were diluted for the hexane (99.9% Lichrosolve, Merck KGaA, Darmstadt, Germany). Identification and quantification regarding potato, Solanum tuberosum, var. Princess, odour was basically mostly carried out in an earlier research (Karlsson et al. 2009). People analyses, in addition to joint fuel chromatography and you may electroantennal recognition (GC-EAD), revealed 17 ingredients from inside the potato headspace that will be antennal effective having T. solanivora. Even more collection throughout the same plant species and you may variety, checked-out with GC-EAD towards the girls, indicated that benzaldehyde, 1-octen-3-ol, 3-octanone, and you will dos-phenylethanol, are also antennal energetic. Therefore, man-made systems ones five substances was in fact right here checked-out with the in earlier times known substances. Ladies insects, old dos–3-time article emergence and you can a dozen–24-h blog post mating were used toward assay. For the first 30 minute pursuing the onset of the brand new photophase, people copulating pairs was basically picked and gently gone to live in a special case. Males and females was in fact partioned into more cages after one done mating feel. Antennae was indeed slashed toward the base and you may climbed that have electroconductive gel (Cefar, Lund, Sweden) when you look at the an effective forked antenna holder (Syntech Gadgets and you will Research, Kirchzarten, Germany) and you can constantly confronted by charcoal-blocked and you will humidified sky (1.5 l minute ?1 ). Brand new odour stimuli was in fact prepared by using ten ?l of every substance and also at for every single amount on items of filter out paper (0.5 ? dos cm), inserted inside a great Pasteur pipette. A smoking device (Syntech stimuli controller CS-55) introduced an excellent 0.5-s long heavens puff that have a flow of 1 ml sek ?step one through the Pasteur pipette and you can to your humidified airstream passageway along side antenna. For every antenna try confronted with several stimulus, in one attention peak, that have been exhibited when you look at the random acquisition, to cease sphericity. New stimuli collection usually become and you can concluded with empty, hexane, and you may female pheromone part (E)-3-dodecenyl acetate, [(E)-3-12:Ac) (Pherobank, Wageningen, Holland >99.6%] (Nesbitt et al. 1985) because resource, since this compound got revealed reproducible EAG responses. For every single stimuli is actually exhibited at the least 1 minute following earlier stimulus to prevent type of your antenna. The utmost amplitude of your EAG answers try recorded and you may analysed into the EAG-modified software (Syntech, EAGPro ver. dos.0).

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